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accuprimetm pfx supermix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher accuprimetm pfx supermix
    Accuprimetm Pfx Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfx+supermix/accuprimetm+pfx+supermix/pmc10483636__pnas__2303574120__sapp-211-19-24
    Average 90 stars, based on 1 article reviews
    accuprimetm pfx supermix - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing
    Article Snippet: .. The hfq gene and ∼1 kb of the upstream flanking region were amplified by using oligonucleotide primers hfq -Up-FLAG-For and hfq -Up-FLAG-Rev (all primers are listed in Table S1 in the supplemental material), B. suis 1330 genomic DNA, and Pfx supermix (Invitrogen). ..

    Article Title: Glutathione Transport: A New Role for PfCRT in Chloroquine Resistance
    Article Snippet: The chimeric genes were expressed under the control of the Pfhsp86 promoter and were generated using the Invitrogen MultiSite Gateway system in combination with the pCHD-3/4 destination vector ( 48 ). .. To generate gene entry clones, the full-length genes for γ gcs and gs were amplified using the primer pairs, defined in , using Pfx SuperMix (Invitrogen) and subcloned according to manufacturer's instructions. .. The Multisite Gateway LR recombination reaction was performed according to the manufacturer's instructions (Invitrogen). table ft1 table-wrap mode=article t1 Table 2. caption a4 Primers Used in This Study Determination of total GSH levels Intracellular GSH levels of saponin-isolated parasites were determined by HPLC ( 51 ).

    Article Title: The stem region of group A transferase is crucial for its specificity, and its alteration promotes heterologous Forssman synthase activity
    Article Snippet: Plasmid pRK5-B4GALT1(1–81)-mTagBFP2 CDS was created by switching the fluorescent protein of pRK5-B4GALT1(1–81)-EGFP by mTagBFP2 from and pmTag-BFP2 (Addgene) plasmid using Bam HI/ Not I restriction sites. .. Tagged mFS, hAT, and hAT constructs were constructed by amplifying the coding regions, eliminating the stop codon, and adding the required tag by PCR amplification using Pfx Supermix (Thermo Scientific), custom oligonucleotides and, as templates, pSG5-mGBGT1, pSG5-ABO(A) and its mutants already available in the laboratory – and subsequently subcloned in pSG5 vector ( Eco RI/ Bam HI). .. New mutants were created by the round-the-horn PCR technique using HS II Phusion mix (Thermo), custom oligonucleotides, and pSG5-ABO(A) as the initial template.

    Article Title: Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins
    Article Snippet: .. Genes were amplified by PCR with Pfx supermix (Invitrogen) and cloned into the pTTQ18-RGSH6 vector as reported ( 7 ). .. Site-directed mutagenesis was conducted by using the QuikChange method as described ( 30 ), but using KOD hot start polymerase (Novagen).

    Mutagenesis:

    Article Title: Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIV SF162P4
    Article Snippet: .. The mutagenesis reaction mixtures consisted of 25 ng of template DNA, a 0.4 M concentration of each mutagenesis primer, and PFX SuperMix (12344-040; Invitrogen Corp, CA). ..

    Article Title: Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIV SF162P4
    Article Snippet: .. The mutagenesis reaction mixtures consisted of 25 ng of template DNA, a 0.4 μM concentration of each mutagenesis primer, and PFX SuperMix (12344-040; Invitrogen Corp, CA). ..

    Concentration Assay:

    Article Title: Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIV SF162P4
    Article Snippet: .. The mutagenesis reaction mixtures consisted of 25 ng of template DNA, a 0.4 M concentration of each mutagenesis primer, and PFX SuperMix (12344-040; Invitrogen Corp, CA). ..

    Article Title: Quaternary Epitope Specificities of Anti-HIV-1 Neutralizing Antibodies Generated in Rhesus Macaques Infected by the Simian/Human Immunodeficiency Virus SHIV SF162P4
    Article Snippet: .. The mutagenesis reaction mixtures consisted of 25 ng of template DNA, a 0.4 μM concentration of each mutagenesis primer, and PFX SuperMix (12344-040; Invitrogen Corp, CA). ..

    Clone Assay:

    Article Title: Glutathione Transport: A New Role for PfCRT in Chloroquine Resistance
    Article Snippet: The chimeric genes were expressed under the control of the Pfhsp86 promoter and were generated using the Invitrogen MultiSite Gateway system in combination with the pCHD-3/4 destination vector ( 48 ). .. To generate gene entry clones, the full-length genes for γ gcs and gs were amplified using the primer pairs, defined in , using Pfx SuperMix (Invitrogen) and subcloned according to manufacturer's instructions. .. The Multisite Gateway LR recombination reaction was performed according to the manufacturer's instructions (Invitrogen). table ft1 table-wrap mode=article t1 Table 2. caption a4 Primers Used in This Study Determination of total GSH levels Intracellular GSH levels of saponin-isolated parasites were determined by HPLC ( 51 ).

    Article Title: Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins
    Article Snippet: .. Genes were amplified by PCR with Pfx supermix (Invitrogen) and cloned into the pTTQ18-RGSH6 vector as reported ( 7 ). .. Site-directed mutagenesis was conducted by using the QuikChange method as described ( 30 ), but using KOD hot start polymerase (Novagen).

    Construct:

    Article Title: The stem region of group A transferase is crucial for its specificity, and its alteration promotes heterologous Forssman synthase activity
    Article Snippet: Plasmid pRK5-B4GALT1(1–81)-mTagBFP2 CDS was created by switching the fluorescent protein of pRK5-B4GALT1(1–81)-EGFP by mTagBFP2 from and pmTag-BFP2 (Addgene) plasmid using Bam HI/ Not I restriction sites. .. Tagged mFS, hAT, and hAT constructs were constructed by amplifying the coding regions, eliminating the stop codon, and adding the required tag by PCR amplification using Pfx Supermix (Thermo Scientific), custom oligonucleotides and, as templates, pSG5-mGBGT1, pSG5-ABO(A) and its mutants already available in the laboratory – and subsequently subcloned in pSG5 vector ( Eco RI/ Bam HI). .. New mutants were created by the round-the-horn PCR technique using HS II Phusion mix (Thermo), custom oligonucleotides, and pSG5-ABO(A) as the initial template.

    Polymerase Chain Reaction:

    Article Title: The stem region of group A transferase is crucial for its specificity, and its alteration promotes heterologous Forssman synthase activity
    Article Snippet: Plasmid pRK5-B4GALT1(1–81)-mTagBFP2 CDS was created by switching the fluorescent protein of pRK5-B4GALT1(1–81)-EGFP by mTagBFP2 from and pmTag-BFP2 (Addgene) plasmid using Bam HI/ Not I restriction sites. .. Tagged mFS, hAT, and hAT constructs were constructed by amplifying the coding regions, eliminating the stop codon, and adding the required tag by PCR amplification using Pfx Supermix (Thermo Scientific), custom oligonucleotides and, as templates, pSG5-mGBGT1, pSG5-ABO(A) and its mutants already available in the laboratory – and subsequently subcloned in pSG5 vector ( Eco RI/ Bam HI). .. New mutants were created by the round-the-horn PCR technique using HS II Phusion mix (Thermo), custom oligonucleotides, and pSG5-ABO(A) as the initial template.

    Article Title: Transcriptomic and biochemical analyses identify a family of chlorhexidine efflux proteins
    Article Snippet: .. Genes were amplified by PCR with Pfx supermix (Invitrogen) and cloned into the pTTQ18-RGSH6 vector as reported ( 7 ). .. Site-directed mutagenesis was conducted by using the QuikChange method as described ( 30 ), but using KOD hot start polymerase (Novagen).

    Article Title: Engineering Hyperthermophilic Archaeon Pyrococcus furiosus to Overproduce Its Cytoplasmic [NiFe]-Hydrogenase
    Article Snippet: .. PCR products were purified using a commercial extraction protocol (Stratagene, Santa Clara, CA) and were used in overlapping PCR reactions using the Pfx supermix (Invitrogen). ..

    Purification:

    Article Title: Engineering Hyperthermophilic Archaeon Pyrococcus furiosus to Overproduce Its Cytoplasmic [NiFe]-Hydrogenase
    Article Snippet: .. PCR products were purified using a commercial extraction protocol (Stratagene, Santa Clara, CA) and were used in overlapping PCR reactions using the Pfx supermix (Invitrogen). ..

    Extraction:

    Article Title: Engineering Hyperthermophilic Archaeon Pyrococcus furiosus to Overproduce Its Cytoplasmic [NiFe]-Hydrogenase
    Article Snippet: .. PCR products were purified using a commercial extraction protocol (Stratagene, Santa Clara, CA) and were used in overlapping PCR reactions using the Pfx supermix (Invitrogen). ..

    Plasmid Preparation:

    Article Title: The stem region of group A transferase is crucial for its specificity, and its alteration promotes heterologous Forssman synthase activity.
    Article Snippet: Plasmid pRK5-B4GALT1(1– 81)-mTagBFP2 CDS was created by switching the fluorescent protein of pRK5-B4GALT1(1–81)-EGFP58 by mTagBFP2 from and pmTag-BFP2 (Addgene) plasmid using BamHI/NotI restriction sites. .. Tagged mFS, hAT, and hAT constructs were constructed by amplifying the coding regions, eliminating the stop codon, and adding the required tag by PCR amplification using Pfx Supermix (Thermo Scientific), custom oligonucleotides and, as templates, pSG5-mGBGT1, pSG5-ABO(A) and its mutants already available in the laboratory12–14 and subsequently subcloned in pSG5 vector (EcoRI/BamHI). .. New mutants were created by the round-the-horn PCR technique59 using HS II Phusion mix (Thermo), custom oligonucleotides, and pSG5-ABO(A) as the initial template.



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